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Molecular characterization and effectiveness cross-protection of weak strains against super-infection malignant strains Cucumber mosaic virus (CMV) on cayenne pepper (Capsicum annuum L.) in Lampung, Indonesia

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA · Vol. 25 No. 1 (2025) · pp. 63–75

DOI
10.23960/jhptt.12563-75
ISSN
2461-0399, 1411-7525
Published
2025-02-22
Publisher
Universitas Lampung
Language
eng
License
creativecommons.org/licenses/by-nc/4.0

Abstract

Cayenne pepper (Capsicum annuum L.) is a horticultural crop of significant economic importance. One of the major threats to its cultivation is the Cucumber mosaic virus (CMV), which can cause severe yield losses and crop failures. This study aimed to isolate weak CMV strains from natural populations of cayenne pepper, characterize their molecular properties, and evaluate the effectiveness of cross-protection conferred by weak strains against superinfection by virulent CMV strains. The research was conducted in three stages: virus isolation, molecular characterization, and evaluation of cross-protection effectiveness. Data analysis was performed using the MEGA v.11.0.11 software and analysis of variance (ANOVA). The findings identified the C1 isolate as a highly virulent CMV strain. Molecular analysis of naturally infected samples confirmed the presence of CMV through the amplification of specific DNA bands. Inoculation tests revealed distinct differences between weak and malignant strains, particularly in symptom severity and molecular characteristics, such as genetic distance. Furthermore, the weak strains demonstrated a protective effect, significantly reducing the infection rate of virulent CMV strains. This was evidenced by variations in symptom expression, disease severity, plant resistance, chlorophyll levels, carbohydrate content, and peroxidase enzyme activity. These findings highlight the potential application of weak CMV strains in developing disease management strategies for cayenne pepper cultivation.

Keywords

Collaboration network of the authors

40 authors · 92 co-authorship links · showing the 40 most connected

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  • Lampung University
  • State University of Semarang
  • Institut Teknologi Sepuluh Nopember
  • Universitas Halu Oleo
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Rike Puspitasari TaminAffiliation unknown3
Riana AnggrainiAffiliation unknown2
Fazriyas FazriyasAffiliation unknown2
Joko PrasetyoInstitut Teknologi Sepuluh Nopember2
Lestari WibowoAffiliation unknown2
Radix SuharjoAffiliation unknown2
Desma AnggrainiAffiliation unknown2
Mohammad HoesainAffiliation unknown2
Fariz Kustiawan AlfarisyAffiliation unknown2
Ankardiansyah Pandu PradanaAffiliation unknown2
Asrul SaniUniversitas Halu Oleo1
Muhammad SudiaAffiliation unknown1
Emantis RosaLampung University1
Supiyanto SupiyantoLampung University1
Nismah NukmalLampung University1
Nabela H.N. HarfianiLampung University1
Mahfut MahfutLampung University1
Endang NurcahyaniLampung University1
Akhmad NurdinAffiliation unknown1
Iqbal ZulfikarAffiliation unknown1
Angger Bagus PrasetiyoAffiliation unknown1
Ahmad SofwanAffiliation unknown1
Eka HaryaniAffiliation unknown1
Ihsan Nuril HikamAffiliation unknown1
Asep SutarmanAffiliation unknown1
Zaharuddin ZaharuddinAffiliation unknown1
Thriwaty ArsalAffiliation unknown1
Martitah MartitahAffiliation unknown1
Ani RusilowatiState University of Semarang1
Nathan HindartoState University of Semarang1
Eva AchmadAffiliation unknown1
Asrizal PaimanAffiliation unknown1
Marwoto MarwotoAffiliation unknown1
Hamzah HamzahAffiliation unknown1
Maria UlfaAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Molecular characterization and effectiveness cross-protection of weak strains against super-infection malignant strains Cucumber mosaic virus (CMV) on cayenne pepper (Capsicum annuum L.) in Lampung, Indonesia</dc:title>
	<dc:creator xml:lang="en">Mahfut, Mahfut</dc:creator>
	<dc:creator xml:lang="en">Sari, Mai</dc:creator>
	<dc:creator xml:lang="en">Akin, Hasriadi Mat</dc:creator>
	<dc:creator xml:lang="en">Wahyuningsih, Sri</dc:creator>
	<dc:creator xml:lang="en">Irawan, Bambang</dc:creator>
	<dc:subject xml:lang="en">cayenne pepper</dc:subject>
	<dc:subject xml:lang="en">cross-protection</dc:subject>
	<dc:subject xml:lang="en">Cucumber mosaic virus (CMV)</dc:subject>
	<dc:subject xml:lang="en">molecular characterization</dc:subject>
	<dc:subject xml:lang="en">weak strains</dc:subject>
	<dc:description xml:lang="en">Cayenne pepper (Capsicum annuum L.) is a horticultural crop of significant economic importance. One of the major threats to its cultivation is the Cucumber mosaic virus (CMV), which can cause severe yield losses and crop failures. This study aimed to isolate weak CMV strains from natural populations of cayenne pepper, characterize their molecular properties, and evaluate the effectiveness of cross-protection conferred by weak strains against superinfection by virulent CMV strains. The research was conducted in three stages: virus isolation, molecular characterization, and evaluation of cross-protection effectiveness. Data analysis was performed using the MEGA v.11.0.11 software and analysis of variance (ANOVA). The findings identified the C1 isolate as a highly virulent CMV strain. Molecular analysis of naturally infected samples confirmed the presence of CMV through the amplification of specific DNA bands. Inoculation tests revealed distinct differences between weak and malignant strains, particularly in symptom severity and molecular characteristics, such as genetic distance. Furthermore, the weak strains demonstrated a protective effect, significantly reducing the infection rate of virulent CMV strains. This was evidenced by variations in symptom expression, disease severity, plant resistance, chlorophyll levels, carbohydrate content, and peroxidase enzyme activity. These findings highlight the potential application of weak CMV strains in developing disease management strategies for cayenne pepper cultivation.</dc:description>
	<dc:publisher xml:lang="en">Universitas Lampung</dc:publisher>
	<dc:date>2025-02-22</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/815</dc:identifier>
	<dc:identifier>10.23960/jhptt.12563-75</dc:identifier>
	<dc:source xml:lang="en">Jurnal Hama dan Penyakit Tumbuhan Tropika; Vol. 25 No. 1 (2025): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PESTS AND DISEASES; 63-75</dc:source>
	<dc:source>2461-0399</dc:source>
	<dc:source>1411-7525</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/815/643</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Jurnal Hama dan Penyakit Tumbuhan Tropika</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>