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Primordial Germ Cells Quality of Chicken Embryo from Post Cryopreservation Blood Samples

Jurnal Medik Veteriner · Vol. 7 No. 2 (2024) · pp. 292–299

DOI
10.20473/jmv.vol7.iss2.2024.292-299
ISSN
2581-012X, 2615-7497
Published
2024-10-04
Publisher
Universitas Airlangga
Language
eng
License
creativecommons.org/licenses/by-nc-sa/4.0

Abstract

The optimal cellular reservoir for the conservation and propagation of endangered poultry is acknowledged to be primordial germ cells (PGCs). This study aimed to evaluate and compare the efficacy of cryopreserving PGCs derived from purified sources and blood samples. The experimental design encompassed two treatment groups, each iterated six replication. Blood samples that had been filled with 500 µL of phosphate-buffered saline without Ca2+ and Mg2+ (PBS) underwent centrifugation at 1200 rpm for six minutes. The supernatant was discarded and the pellet was mixed with 500 µL 10% FBS-PBS and was then subdivided for cryopreservation and PGCs purification. Cryopreservation included the controlled freezing of blood and purified PGCs samples in Nalgene cryogenic vials with the incorporation of banker cells. Following thawing, statistical analyses revealed no significant variance in the mean PGCs count between purified PGCs and blood samples (p > 0.05). The percentages of PGCs mortality and the recovery rate demonstrated statistical significance (p < 0.05). Further investigations indicated that cryopreserving blood samples significantly increased the recovery rate of PGCs while decreasing post-thaw mortality. Morphology of PGCs from blood cryopreservation showed similar morphology to PGCs in general. The potential application of cell-based cryo-banking will contribute to the preservation of poultry genetic diversity for sustainability and adaptation to future poultry demands.

Keywords

Collaboration network of the authors

40 authors · 98 co-authorship links · showing the 40 most connected

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Co-authorOrganisationJoint articles
Lilis WijayantiAffiliation unknown4
Martha Irene KartasuryaAffiliation unknown3
Hasna Izdihar LatifahAffiliation unknown3
Siti FatimahAffiliation unknown3
Rusdarti RusdartiAffiliation unknown2
I Made SudanaAffiliation unknown2
Jihan Avie YusrinaAffiliation unknown2
Erna Dwi NugrainiAffiliation unknown2
Laila Ngindana ZulfaAffiliation unknown2
Ahmad SyauqyAffiliation unknown2
Sri Achadi NugraheniAffiliation unknown2
Yendraliza YendralizaAffiliation unknown2
S SopiyanaAffiliation unknown2
Ria Sari Gail SianturiAffiliation unknown2
Sitti Wirdhana AhmadAffiliation unknown1
Indrawati - IndrawatiAffiliation unknown1
SyarifahAffiliation unknown1
Intan JuniceAffiliation unknown1
Fatimah IlmaAffiliation unknown1
Latifah DhiahanaAffiliation unknown1
Linda Indiyarti PutriAffiliation unknown1
Ummu Jauharin FardaAffiliation unknown1
Indriyanto IndriyantoAffiliation unknown1
Sestri Indah PebriantiAffiliation unknown1
Edy PurwantoAffiliation unknown1
Mungin Eddy WibowoAffiliation unknown1
ZukhairaAffiliation unknown1
Nafis Azmi AmrullahAffiliation unknown1
Bayu SujadmikoAffiliation unknown1
Rohaini RohainiAffiliation unknown1
Nobuhide OtomoAffiliation unknown1
Ikhsan SetiawanAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/JMV/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Primordial Germ Cells Quality of Chicken Embryo from Post Cryopreservation Blood Samples</dc:title>
	<dc:creator>Azizah, Nurul</dc:creator>
	<dc:creator>Kostaman, Tatan</dc:creator>
	<dc:creator>Tribudi, Yuli Arif</dc:creator>
	<dc:creator>Kusumaningrum, Diana Andrianita</dc:creator>
	<dc:creator>Suyatno, Suyatno</dc:creator>
	<dc:creator>Muttaqin, Zultinur</dc:creator>
	<dc:creator>Koswara, Eko</dc:creator>
	<dc:creator>Gunawan, Muhammad</dc:creator>
	<dc:subject xml:lang="en-US">blood</dc:subject>
	<dc:subject xml:lang="en-US">cryopreservation</dc:subject>
	<dc:subject xml:lang="en-US">endangered poultry</dc:subject>
	<dc:subject xml:lang="en-US">primordial germ cells</dc:subject>
	<dc:subject xml:lang="en-US">purified</dc:subject>
	<dc:description xml:lang="en-US">The optimal cellular reservoir for the conservation and propagation of endangered poultry is acknowledged to be primordial germ cells (PGCs). This study aimed to evaluate and compare the efficacy of cryopreserving PGCs derived from purified sources and blood samples. The experimental design encompassed two treatment groups, each iterated six replication. Blood samples that had been filled with 500 µL of phosphate-buffered saline without Ca2+ and Mg2+ (PBS) underwent centrifugation at 1200 rpm for six minutes. The supernatant was discarded and the pellet was mixed with 500 µL 10% FBS-PBS and was then subdivided for cryopreservation and PGCs purification. Cryopreservation included the controlled freezing of blood and purified PGCs samples in Nalgene cryogenic vials with the incorporation of banker cells. Following thawing, statistical analyses revealed no significant variance in the mean PGCs count between purified PGCs and blood samples (p &amp;gt; 0.05). The percentages of PGCs mortality and the recovery rate demonstrated statistical significance (p &amp;lt; 0.05). Further investigations indicated that cryopreserving blood samples significantly increased the recovery rate of PGCs while decreasing post-thaw mortality. Morphology of PGCs from blood cryopreservation showed similar morphology to PGCs in general. The potential application of cell-based cryo-banking will contribute to the preservation of poultry genetic diversity for sustainability and adaptation to future poultry demands.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Airlangga</dc:publisher>
	<dc:date>2024-10-04</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>text/xml</dc:format>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JMV/article/view/55140</dc:identifier>
	<dc:identifier>10.20473/jmv.vol7.iss2.2024.292-299</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Medik Veteriner; Vol. 7 No. 2 (2024): October; 292-299</dc:source>
	<dc:source>2581-012X</dc:source>
	<dc:source>2615-7497</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/55140/31253</dc:relation>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/55140/29888</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2024 Nurul Azizah, Tatan Kostaman, Yuli Arif Tribudi, Diana Andrianita Kusumaningrum, Suyatno Suyatno, Zultinur Muttaqin, Eko Koswara, Muhammad Gunawan</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>