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Identifikasi Spesies Ikan Hiu dan Pari pada Produk Olahan Ikan Asap dengan Metode DNA Barcoding: Identification of Shark and Sting Fish Species in Smoked Fish Processed Products Using The DNA Barcoding Method

Jurnal Pengolahan Hasil Perikanan Indonesia · Vol. 25 No. 1 (2022) · pp. 163–171

DOI
10.17844/jphpi.v25i1.38518
ISSN
2354-886X, 2303-2111
Published
2022-04-29
Publisher
Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)
Language
ind

Abstract

Indonesian

Ikan hiu dan pari adalah kelompok ikan yang diatur perdagangannya di tingkat lokal dan internasional. Tingginya permintaan produk hiu di pasaran dapat berakibat pada kelangkaan spesies-spesies hiu tertentu di alam. Autentikasi pada produk-produk olahan hiu diperlukan untuk mendeteksi jenis spesies hiu yang digunakan pada suatu produk. Tujuan dari penelitian ini yaitu mengidentifikasi spesies ikan hiu dan pari asap yang berasal dari Perairan Utara Pulau Jawa, Tegal, Jawa Tengah dengan metode DNA barcoding. Metode penelitian terdiri dari pengumpulan sampel, isolasi DNA, Ekstraksi DNA, Konsentrasi dan Kemurnian DNA, amplifikasi dan pengurutan PCR, Elektroforesis, Sanger Sequencing, Pengujian PCR dengan marka molekuler COI, dan analisis data bioinformatika. Hasil identifikasi spesies dari produk olahan ikan pari dan hiu asap adalah terdeteksi menggunakan hiu di antaranya spesies Carcharhinus brevipinna, Telatrygon zugei, Rhizoprionodon oligolinx, Himantura uarnacoides, Maculabatis macrura, Hydrolagus novaezealandiae, dan Rhynchobatus australiae. Rhynchobatus australiae dan Carcharhinus brevipinna adalah spesies yang terdaftar sebagai hiu dilindungi dalam CITES II. Sekuen yang didapatkan memiliki persentase kemiripan antara 99,84-100% dengan sekuen yang terdapat pada GenBank.

English

Sharks and rays are a group of fish whose trade is regulated both locally and internationally. The high demand for shark products in the market can result in the scarcity of certain shark species in the wild. Authentication on processed shark products is needed to detect the type of shark species used in a product. The purpose of this study was to identify species of sharks and rays in processed smoked products originating from the PANTURA, Tegal Jawa Tengah using the DNA barcoding method. The research method consisted of sample collection, DNA isolation, DNA extraction, DNA concentration and purity, PCR amplification and sequencing, Electrophoresis, Sanger Sequencing, PCR testing with COI molecular marker, and analysis of bioinformatics data. Species identification results from processed products of stingrays and smoked sharks were detected using sharks, including Carcharhinus brevipinna, Telatrygon zugei, Rhizoprionodon oligolinx, Himantura uarnacoides, Maculabatis macrura, Hydrolagus novaezealandiae, and Rhynchobatus australiae. Rhynchobatus australiae and Carcharhinus brevipinna are species listed as a protected shark in CITES II. The sequences obtained have a similarity percentage between 99.84-100% with the sequences found in GenBank.

Keywords

Collaboration network of the authors

40 authors · 136 co-authorship links · showing the 40 most connected

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  • IPB University
  • Hasanuddin University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
nurjanah nurjanahAffiliation unknown11
Mala NurilmalaAffiliation unknown11
Tati NurhayatiAffiliation unknown10
NurjanahAffiliation unknown5
Ruddy SuwandiAffiliation unknown5
Agoes Mardiono JacoebAffiliation unknown5
Rizsa Mustika PertiwiAffiliation unknown4
Puji RiantiAffiliation unknown3
Anggrei Viona SeulalaeAffiliation unknown3
N. NurjanahAffiliation unknown2
Chairun NufusAffiliation unknown2
Agoes Mardiono JacoebAffiliation unknown2
Agoes M.JacoebAffiliation unknown2
Aninditya Artina SetiaputriAffiliation unknown2
RamlanAffiliation unknown2
Roza YusfiandayaniAffiliation unknown1
M. Fedi A. SonditaAffiliation unknown1
Diana Ningdya LihuanaAffiliation unknown1
Ari Elisa RatihAffiliation unknown1
Shabrina AuliaAffiliation unknown1
Firda Agnes MonicaAffiliation unknown1
Agus YuliantoAffiliation unknown1
Chandabalo ChandabaloAffiliation unknown1
Siti Nur HakimahAffiliation unknown1
Ismi Isti’anahAffiliation unknown1
Kustiariyah TarmanIPB University1
Sugeng Heri SusenoIPB University1
Irzal EffendiIPB University1
Novemi Gita SafitriAffiliation unknown1
Neviaty Putri ZamaniHasanuddin University1
Inna Puspa AyuAffiliation unknown1
Beginer SubhanAffiliation unknown1
Dondy ArafatAffiliation unknown1
Murad Alvian K AdewalAffiliation unknown1
Rosella SilabanAffiliation unknown1
Megana DuanassuryaAffiliation unknown1
Sri Widy YaniAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/jphpi/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="id">Identifikasi Spesies Ikan Hiu dan Pari pada Produk Olahan Ikan Asap dengan Metode DNA Barcoding  : Identification of Shark and Sting Fish Species in Smoked Fish Processed Products Using The DNA Barcoding Method</dc:title>
	<dc:creator xml:lang="id">Alfitri, Misla </dc:creator>
	<dc:creator xml:lang="id">Abdullah, Asadatun</dc:creator>
	<dc:creator xml:lang="id">Nugraha, Roni</dc:creator>
	<dc:creator xml:lang="en">Alfitri, Misla</dc:creator>
	<dc:creator xml:lang="en">Abdullah, Asadatun</dc:creator>
	<dc:creator xml:lang="en">Nugraha, Roni</dc:creator>
	<dc:subject xml:lang="en">authentication</dc:subject>
	<dc:subject xml:lang="en">processed smoked fish</dc:subject>
	<dc:subject xml:lang="en">sequencing</dc:subject>
	<dc:subject xml:lang="id">autentikasi</dc:subject>
	<dc:subject xml:lang="id">olahan ikan asap</dc:subject>
	<dc:subject xml:lang="id">sekuensing</dc:subject>
	<dc:description xml:lang="id">Ikan hiu dan pari adalah kelompok ikan yang diatur perdagangannya di tingkat lokal dan internasional. Tingginya permintaan produk hiu di pasaran dapat berakibat pada kelangkaan spesies-spesies hiu tertentu di alam. Autentikasi pada produk-produk olahan hiu diperlukan untuk mendeteksi jenis spesies hiu yang digunakan pada suatu produk. Tujuan dari penelitian ini yaitu mengidentifikasi spesies ikan hiu dan pari asap yang berasal dari Perairan Utara Pulau Jawa, Tegal, Jawa Tengah dengan metode DNA barcoding. Metode penelitian terdiri dari pengumpulan sampel, isolasi DNA, Ekstraksi DNA, Konsentrasi dan Kemurnian DNA, amplifikasi dan pengurutan PCR, Elektroforesis, Sanger Sequencing, Pengujian PCR dengan marka molekuler COI, dan analisis data bioinformatika. Hasil identifikasi spesies dari produk olahan ikan pari dan hiu asap adalah terdeteksi menggunakan hiu di antaranya spesies Carcharhinus brevipinna, Telatrygon zugei, Rhizoprionodon oligolinx, Himantura uarnacoides, Maculabatis macrura, Hydrolagus novaezealandiae, dan Rhynchobatus australiae. Rhynchobatus australiae dan Carcharhinus brevipinna adalah spesies yang terdaftar sebagai hiu dilindungi dalam CITES II. Sekuen yang didapatkan memiliki persentase kemiripan antara 99,84-100% dengan sekuen yang terdapat pada GenBank.</dc:description>
	<dc:description xml:lang="en">Sharks and rays are a group of fish whose trade is regulated both locally and internationally. The high demand for shark products in the market can result in the scarcity of certain shark species in the wild. Authentication on processed shark products is needed to detect the type of shark species used in a product. The purpose of this study was to identify species of sharks and rays in processed smoked products originating from the PANTURA, Tegal Jawa Tengah using the DNA barcoding method. The research method consisted of sample collection, DNA isolation, DNA extraction, DNA concentration and purity, PCR amplification and sequencing, Electrophoresis, Sanger Sequencing, PCR testing with COI molecular marker, and analysis of bioinformatics data. Species identification results from processed products of stingrays and smoked sharks were detected using sharks, including Carcharhinus brevipinna, Telatrygon zugei, Rhizoprionodon oligolinx, Himantura uarnacoides, Maculabatis macrura, Hydrolagus novaezealandiae, and Rhynchobatus australiae. Rhynchobatus australiae and Carcharhinus brevipinna are&amp;nbsp; species listed as a protected shark in CITES II. The sequences obtained have a similarity percentage between 99.84-100% with the sequences found in GenBank.</dc:description>
	<dc:publisher xml:lang="en">Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)</dc:publisher>
	<dc:date>2022-04-29</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/octet-stream</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/jphpi/article/view/38518</dc:identifier>
	<dc:identifier>10.17844/jphpi.v25i1.38518</dc:identifier>
	<dc:source xml:lang="id">Jurnal Pengolahan Hasil Perikanan Indonesia; Vol 25 No 1 (2022): Jurnal Pengolahan Hasil Perikanan Indonesia 25(1); 163-171</dc:source>
	<dc:source xml:lang="en">Jurnal Pengolahan Hasil Perikanan Indonesia; Vol. 25 No. 1 (2022): Jurnal Pengolahan Hasil Perikanan Indonesia 25(1); 163-171</dc:source>
	<dc:source>2354-886X</dc:source>
	<dc:source>2303-2111</dc:source>
	<dc:language>ind</dc:language>
	<dc:relation>https://journal.ipb.ac.id/jphpi/article/view/38518/23403</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2022 Misla Alfitri, Asadatun Abdullah, Roni Nugraha</dc:rights>
</oai_dc:dc>