Melola Discovery

← Melola Discovery

Journal

Comparison of Eco - Scale Between UV - Vis Spectrophotometry and Thin Layer Chromatography - Densitometry Methods on The Determinination of Retinoic Acid in Facial Cream Preparations

Journal of Science and Technology Research for Pharmacy · Vol. 4 No. 1 (2025) · pp. 15–21

DOI
10.15294/jstrp.v4i1.5415
ISSN
2776-0685
Published
2025-07-10
Publisher
Universitas Negeri Semarang
Language
eng

Abstract

Retinoic acid, a compound derived from vitamin A and its active metabolites, is often found in cream preparations and can be used without a doctor's prescription. This research aims to analyze the retinoic acid content in facial cream preparations and determine the environmental friendliness of the analytical method used. The materials used in this research were PBFI retinoic acid, methanol, TLC plates, n-hexane, acetone, and three facial cream samples. UV - Vis Spectrophotometry and Thin Layer Chromatography - Densitometry are methods used for assay analysis and Eco - Scale is a method for determining greenness. The research found that three cream samples tested positive for retinoic acid, which has been banned by BPOM since 1999. The UV-Vis Spectrophotometry method measured retinoic acid content of 0.103%, 0.266% and 0.256%, with a total score of 24 points. The Thin Layer Chromatography - Densitometry method measured retinoic acid content of 0.0213%, 0.0314%, and 0.027%, with a score of 50 points. It can be concluded that the UV - Vis Spectrophotometry method is more environmentally friendly compared to the Thin Layer Chromatography - Densitometry method based on Eco - Scale analysis.

Keywords

Collaboration network of the authors

40 authors · 97 co-authorship links

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • State University of Semarang
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Agung Tri PrasetyaState University of Semarang5
Eko Budi SusatyoAffiliation unknown3
Wisnu SunartoAffiliation unknown2
Charisma ChalifAffiliation unknown1
Anida Hasna RahmatikaAffiliation unknown1
Sistia Asfa FiqrotinnisaAffiliation unknown1
HarjonoState University of Semarang1
F. ‪Widhi MahatmantiAffiliation unknown1
Riska Putri MawarniAffiliation unknown1
Hanifah Az-ZahraAffiliation unknown1
Erwin Budi PamungkasAffiliation unknown1
Gayuh Ganata Imam SofyanAffiliation unknown1
Riera Asti WulaningrumAffiliation unknown1
Himnil KhusnaAffiliation unknown1
Novilla AnggasariAffiliation unknown1
Winarni PratjojoAffiliation unknown1
Arfah Ratna Puri GustianAffiliation unknown1
Ervina Nur HidayatiAffiliation unknown1
Nova Shintia BokauAffiliation unknown1
Dyah SetyaningrumAffiliation unknown1
Aurelia Anggit Widya MunikaAffiliation unknown1
Endang SusilaningsihState University of Semarang1
Zumrotus Sa’adahAffiliation unknown1
Suryat Hadi KusumaAffiliation unknown1
Amallia KurniawatiAffiliation unknown1
Puji RizqiAffiliation unknown1
Nydia Ashfi AuhaAffiliation unknown1
Nuril HudaAffiliation unknown1
Ridho PrasetyoAffiliation unknown1
Martin SulistyaniAffiliation unknown1
Edy CahyonoState University of Semarang1
Dante AlighiriState University of Semarang1
Tri HandayaniAffiliation unknown1
Nugrahaningsih WHAffiliation unknown1
Adrian Maulana ZakyAffiliation unknown1
Sabila AisyahAffiliation unknown1
Bella PermatasariAffiliation unknown1
Monica AvissaAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/JSTRP/oai, first harvested 5 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Comparison of Eco - Scale Between UV - Vis Spectrophotometry and Thin Layer Chromatography - Densitometry Methods on The Determinination of Retinoic Acid in Facial Cream Preparations</dc:title>
	<dc:creator xml:lang="en">Nur Faida, Zaneta</dc:creator>
	<dc:creator xml:lang="en">Mohammad Alauhdin</dc:creator>
	<dc:subject xml:lang="en">Eco – Scale </dc:subject>
	<dc:subject xml:lang="en">UV – Vis Spectrophotometry</dc:subject>
	<dc:subject xml:lang="en">Thin Layer Chromatography – Densitometry</dc:subject>
	<dc:subject xml:lang="en">Retinoic Acid</dc:subject>
	<dc:subject xml:lang="en">Facial Cream</dc:subject>
	<dc:description xml:lang="en">Retinoic acid, a compound derived from vitamin A and its active metabolites, is often found in cream preparations and can be used without a doctor's prescription. This research aims to analyze the retinoic acid content in facial cream preparations and determine the environmental friendliness of the analytical method used. The materials used in this research were PBFI retinoic acid, methanol, TLC plates, n-hexane, acetone, and three facial cream samples. UV - Vis Spectrophotometry and Thin Layer Chromatography - Densitometry are methods used for assay analysis and Eco - Scale is a method for determining greenness. The research found that three cream samples tested positive for retinoic acid, which has been banned by BPOM since 1999. The UV-Vis Spectrophotometry method measured retinoic acid content of 0.103%, 0.266% and 0.256%, with a total score of 24 points. The Thin Layer Chromatography - Densitometry method measured retinoic acid content of 0.0213%, 0.0314%, and 0.027%, with a score of 50 points. It can be concluded that the UV - Vis Spectrophotometry method is more environmentally friendly compared to the Thin Layer Chromatography - Densitometry method based on Eco - Scale analysis.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2025-07-10</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/JSTRP/article/view/5415</dc:identifier>
	<dc:identifier>10.15294/jstrp.v4i1.5415</dc:identifier>
	<dc:source xml:lang="en">Journal of Science and Technology Research for Pharmacy; Vol. 4 No. 1 (2024): Journal of Science and Technology Research for Pharmacy; 15-21</dc:source>
	<dc:source>2776-0685</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/JSTRP/article/view/5415/4340</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Journal of Science and Technology Research for Pharmacy</dc:rights>
</oai_dc:dc>