Melola Discovery

← Melola Discovery

Journal

Optimization of The Concentration of Precipitation Reagent in Cyclamate Analysis and Determination of Cyclamate Contents in Jamu

Journal of Science and Technology Research for Pharmacy · Vol. 3 No. 2 (2025) · pp. 20–27

DOI
10.15294/jstrp.v3i2.9329
ISSN
2776-0685
Published
2025-07-10
Publisher
Universitas Negeri Semarang
Language
eng

Abstract

: Sodium cyclamate is one of the food additives sweeteners that are often added to food products including jamu. It has a low price and a taste 30 - 50 times sweeter than natural sugar. It is known that excessive & longterm use of sodium cyclamate can cause adverse health effects. Currently, the standard method used to detect the presence of cyclamate refers to the precipitation method of SNI 01-2893. However, the level of sensitivity of the method is unknown. This study aims to determine the sensitivity of the precipitation method of SNI 01-2893, as well as to optimize reagents that have better sensitivity to be used as a reference for cyclamate precipitation reagents and applied to measure cyclamate in jamu. The determination the method’s sensitivity is based on the smallest concentration of cyclamate standard that can be detected by the method. Optimization of the precipitation reagent was carried out on 6 variations of precipitation reagent concentration to obtain a new formula that has a better sensitivity than the SNI 01-2893 method. Jamu samples were tested using the new optimized reagent formula. The result showed the detection limit of the of SNI 01-2893 method is 5 ppm. Optimization of the precipitation reagent formula with the composition of HCl 10%, BaCl2 10%, and NaNO2 20% showed as the highest sensitivity. It was marked by an increase in the amount of precipitate formed and had a detection limit of up to 0.9 ppm. Based on testing using the new reagent, 9 out of 10 jamu samples were positive for cyclamate. The cyclamate levels of the 9 jamu samples were measured using the spectrophotometric method, the results showed cyclamate levels are in the range of 4.3 - 6.32 mg/kg sample and meeting BPOM requirements.

Keywords

Collaboration network of the authors

36 authors · 72 co-authorship links

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • State University of Semarang
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Agung Tri PrasetyaState University of Semarang5
Eko Budi SusatyoAffiliation unknown3
Wisnu SunartoAffiliation unknown2
Charisma ChalifAffiliation unknown1
Zaneta Nur FaidaAffiliation unknown1
Anida Hasna RahmatikaAffiliation unknown1
Agus SuciptoAffiliation unknown1
Wahida RahmawatiAffiliation unknown1
Hanifah -Affiliation unknown1
Fadila IskandarAffiliation unknown1
HarjonoState University of Semarang1
F. ‪Widhi MahatmantiAffiliation unknown1
Riska Putri MawarniAffiliation unknown1
Hanifah Az-ZahraAffiliation unknown1
Erwin Budi PamungkasAffiliation unknown1
Gayuh Ganata Imam SofyanAffiliation unknown1
Riera Asti WulaningrumAffiliation unknown1
Himnil KhusnaAffiliation unknown1
Novilla AnggasariAffiliation unknown1
Winarni PratjojoAffiliation unknown1
Arfah Ratna Puri GustianAffiliation unknown1
Ervina Nur HidayatiAffiliation unknown1
Nova Shintia BokauAffiliation unknown1
Dyah SetyaningrumAffiliation unknown1
Aurelia Anggit Widya MunikaAffiliation unknown1
Endang SusilaningsihState University of Semarang1
Zumrotus Sa’adahAffiliation unknown1
Suryat Hadi KusumaAffiliation unknown1
Amallia KurniawatiAffiliation unknown1
Puji RizqiAffiliation unknown1
Nydia Ashfi AuhaAffiliation unknown1
Nuril HudaAffiliation unknown1
Ridho PrasetyoAffiliation unknown1
Martin SulistyaniAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/JSTRP/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Optimization of The Concentration of Precipitation Reagent in Cyclamate Analysis and Determination of Cyclamate Contents in Jamu</dc:title>
	<dc:creator xml:lang="en">Sistia Asfa Fiqrotinnisa</dc:creator>
	<dc:creator xml:lang="en">Mohammad Alauhdin</dc:creator>
	<dc:subject xml:lang="en">Jamu</dc:subject>
	<dc:subject xml:lang="en">Precipitation Method</dc:subject>
	<dc:subject xml:lang="en">Sodium Cyclamate</dc:subject>
	<dc:description xml:lang="en">: Sodium cyclamate is one of the food additives sweeteners that are often added to food products including jamu. It has a low price and a taste 30 - 50 times sweeter than natural sugar. It is known that excessive &amp;amp; longterm use of sodium cyclamate can cause adverse health effects. Currently, the standard method used to detect the presence of cyclamate refers to the precipitation method of SNI 01-2893. However, the level of sensitivity of the method is unknown. This study aims to determine the sensitivity of the precipitation method of SNI 01-2893, as well as to optimize reagents that have better sensitivity to be used as a reference for cyclamate precipitation reagents and applied to measure cyclamate in jamu. The determination the method’s sensitivity is based on the smallest concentration of cyclamate standard that can be detected by the method. Optimization of the precipitation reagent was carried out on 6 variations of precipitation reagent concentration to obtain a new formula that has a better sensitivity than the SNI 01-2893 method. Jamu samples were tested using the new optimized reagent formula. The result showed the detection limit of the of SNI 01-2893 method is 5 ppm. Optimization of the precipitation reagent formula with the composition of HCl 10%, BaCl2 10%, and NaNO2 20% showed as the highest sensitivity. It was marked by an increase in the amount of precipitate formed and had a detection limit of up to 0.9 ppm. Based on testing using the new reagent, 9 out of 10 jamu samples were positive for cyclamate. The cyclamate levels of the 9 jamu samples were measured using the spectrophotometric method, the results showed cyclamate levels are in the range of 4.3 - 6.32 mg/kg sample and meeting BPOM requirements.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2025-07-10</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/JSTRP/article/view/9329</dc:identifier>
	<dc:identifier>10.15294/jstrp.v3i2.9329</dc:identifier>
	<dc:source xml:lang="en">Journal of Science and Technology Research for Pharmacy; Vol. 3 No. 2 (2023): Journal of Science and Technology Research for Pharmacy; 20-27</dc:source>
	<dc:source>2776-0685</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/JSTRP/article/view/9329/4338</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Journal of Science and Technology Research for Pharmacy</dc:rights>
</oai_dc:dc>