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Production, Characterization, and Molecular Phylogenetic Analysis of Phytase from Aspergillus niger Isolates of an Indonesia Origin

HAYATI Journal of Biosciences · Vol. 29 No. 4 (2022) · hlm. 468–478

DOI
10.4308/hjb.29.4.468-478
ISSN
2086-4094, 1978-3019
Terbit
2022-04-06
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

This research aimed at analyzing the phytase from fungal isolates SB1, SB2, BS, and WF produced in cornstarch with glucose medium (CS+Glu) as carbon sources and Potatoes dextrose broth (PDB). The activity of phytase from isolates SB1, SB2, BS, and WF produced in CS+Glu medium was 2.97 UmL-1, 2.87 UmL-1, 3.18 UmL-1, and 4.37 UmL-1, respectively, while the activity of phytases was 2.07 UmL-1, 2.17 UmL-1, 2.22 UmL-1, and 2.78 UmL-1 respectively in PDB medium. The optimal temperature of SB1 and WF phytase was 40°C, while SB2 and BS were 50°C and 60°C, respectively. The optimal pH of SB1 and WF phytase was 5.0, while SB2 and BS phytase were 6.0, and 4.0 respectively. 18S rRNA gene analysis revealed that SB1 was 99% identical to Aspergillus niger ANTS (KY825168.1), SB2, BS, and WF were 99% identical to A. niger Moriga leaf (MG889596.1). Multiple sequences and phylogenetic analysis of phytase gene showed that phyA_SB1 and phyA_SB2 were 98% homology with A. ficuum (AAB26466), 97% with A. niger (ADP05107) while phyA_WF was 99% with A. ochraceoroseus (PLB29348), 98% with A. niger (ADP05105). The deduced proteins contain conserved motifs RHGARYPTD at N-terminal while lacking HD motif at C-terminal. These phytases were in the same cluster with Aspergillus sp. phytase A indicating that they belong to Histidine Acid Phosphatases (HAP) family.

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  • State University of Semarang
  • Universiti Brunei Darussalam
  • National University of Malaysia
  • Indonesian Institute of Sciences
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Rekan penulisOrganisasiArtikel bersama
Suranto SurantoSebelas Maret University4
Muhammad AkhyarSebelas Maret University3
Ratna SetyaningsihAfiliasi tidak diketahui3
Idam Ragil Widianto AtmojoSebelas Maret University2
Riezky Maya ProbosariSebelas Maret University2
Ashadi AshadiSebelas Maret University2
S. SutiknoState University of Semarang2
Sri WidoretnoSebelas Maret University2
Sri DwiastutiSebelas Maret University2
Siti Lusi Arum SariAfiliasi tidak diketahui2
Prabang SetyonoSebelas Maret University2
Basori BasoriAfiliasi tidak diketahui2
Wiranto WirantoAfiliasi tidak diketahui2
Mohammad MasykuriSebelas Maret University2
Roy ArdiansyahAfiliasi tidak diketahui1
Dwi Yuniasih SaputriAfiliasi tidak diketahui1
Roslinawati Mohd RoslanUniversiti Brunei Darussalam1
Lilia HalimNational University of Malaysia1
Dini Yuli SaputriSebelas Maret University1
R. EtvianaSebelas Maret University1
Bertha WikaraSebelas Maret University1
S. SutarnoSebelas Maret University1
Baskoro Adi PrayitnoSebelas Maret University1
Afandi AfandiSebelas Maret University1
Nunuk SuryaniSebelas Maret University1
P. ParminState University of Semarang1
Fidia FibrianaState University of Semarang1
Annisa Nur KhasanahSebelas Maret University1
Ade Cyntia PritasariSebelas Maret University1
Parmin ParminState University of Semarang1
Sutarno SutarnoIndonesian Institute of Sciences1
Sigit PrastowoAfiliasi tidak diketahui1
Shavya Sarah SavieraAfiliasi tidak diketahui1
Rebecca VanessaAfiliasi tidak diketahui1
Galih PambukoAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Production, Characterization, and Molecular Phylogenetic Analysis of Phytase from Aspergillus niger Isolates of an Indonesia Origin</dc:title>
	<dc:creator xml:lang="en">Mkumbe, Baraka Stewart</dc:creator>
	<dc:creator xml:lang="en">Sajidan, Sajidan</dc:creator>
	<dc:creator xml:lang="en">Pangastuti, Artini</dc:creator>
	<dc:creator xml:lang="en">Susilowati, Ari</dc:creator>
	<dc:creator xml:lang="en">Greiner, Ralf</dc:creator>
	<dc:description xml:lang="en">This research aimed at analyzing the phytase from fungal isolates SB1, SB2, BS, and WF produced in cornstarch with glucose medium (CS+Glu) as carbon sources and Potatoes dextrose broth (PDB). The activity of phytase from isolates SB1, SB2, BS, and WF produced in CS+Glu medium was 2.97 UmL-1, 2.87 UmL-1, 3.18 UmL-1, and 4.37 UmL-1, respectively, while the activity of phytases was 2.07 UmL-1, 2.17 UmL-1, 2.22 UmL-1, and 2.78 UmL-1 respectively in PDB medium. The optimal temperature of SB1 and WF phytase was 40°C, while SB2 and BS were 50°C and 60°C, respectively. The optimal pH of SB1 and WF phytase was 5.0, while SB2 and BS phytase were 6.0, and 4.0 respectively. 18S rRNA gene analysis revealed that SB1 was 99% identical to Aspergillus niger ANTS (KY825168.1), SB2, BS, and WF were 99% identical to A. niger Moriga leaf (MG889596.1). Multiple sequences and phylogenetic analysis of phytase gene showed that phyA_SB1 and phyA_SB2 were 98% homology with A. ficuum (AAB26466), 97% with A. niger (ADP05107) while phyA_WF was 99% with A. ochraceoroseus (PLB29348), 98% with A. niger (ADP05105). The deduced proteins contain conserved motifs RHGARYPTD at N-terminal while lacking HD motif at C-terminal. These phytases were in the same cluster with Aspergillus sp. phytase A indicating that they belong to Histidine Acid Phosphatases (HAP) family.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2022-04-06</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/40622</dc:identifier>
	<dc:identifier>10.4308/hjb.29.4.468-478</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 29 No. 4 (2022): July 2022; 468-478</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/40622/23267</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2022 Baraka Stewart Mkumbe, Sajidan Sajidan, Artini Pangastuti, Ari Susilowati, Ralf Greiner</dc:rights>
</oai_dc:dc>