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Karakterisasi DNA Mikrobiota Usus Bayi pada Persalinan Normal yang diberi ASI dan Susu Formula

Media Kesehatan Masyarakat Indonesia · Vol. 16 No. 1 (2020) · pp. 116–126

DOI
10.30597/mkmi.v16i1.9050
ISSN
2356-4067, 0216-2482
Published
2020-03-31
Publisher
Faculty of Public Health, Hasanuddin University, Makassar
Language
en
License
creativecommons.org/licenses/by-nc-sa/4.0

Abstract

Breast milk is the best source of nutrition because it contains a variety of healthy compounds and immunity. Several studies of gut microbiota has proven its important role in the development of the immune system. This study aims to characterize the gut microbiota of babies born normally with breastfeeding and formula milk. This research using cross sectional study design with purposive sampling as sampling technique. Samples used were babies born normally as many as 30 infants that divided into two groups namely infants who given breast milk and combinated breastfeeding and formulamilk of 15 infants each. Stool samples were taken by swab technique, the DNA was extracted using a DNA kit continued DNA amplification by PCR with 16SrRNA universal primers then DNA sequencing. Data from DNA sequencer analized using a software of MEGA5 and BLAST attained on the NCBI link. The result showed that the group of infants who were breastfed plus formula milk had more varied intestinal microbiota than the group of infants who were only given breast milk. It can be concluded that intestinal microbiota that dominates the intestines of infants who are given breast milk can be a probiotic in the growth of the baby and the development of the immune system.

Keywords

Collaboration network of the authors

24 authors · 45 co-authorship links

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Halu Oleo
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Rizal Adi SaputraUniversitas Halu Oleo1
Sutardi SutardiUniversitas Halu Oleo1
Nani YuniarUniversitas Halu Oleo1
Devi Safitri EffendyAffiliation unknown1
Luh SukariasihUniversitas Halu Oleo1
La TahangAffiliation unknown1
Nur AsyikUniversitas Halu Oleo1
Ansharullah AnsharullahUniversitas Halu Oleo1
Warsono WarsonoAffiliation unknown1
Rasheed AhmadAffiliation unknown1
Anwar MallongiAffiliation unknown1
Stang Abdul RahmanAffiliation unknown1
Ary HandayaniAffiliation unknown1
Siti FadlilahAffiliation unknown1
Adi SuciptoAffiliation unknown1
Nazwar Hamdani RahilAffiliation unknown1
Muhammad DarwisAffiliation unknown1
Inriati LewaAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/mkmi/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Karakterisasi DNA Mikrobiota Usus Bayi pada Persalinan Normal yang diberi ASI dan Susu Formula</dc:title>
	<dc:creator xml:lang="en">Kamaruddin, Mudyawati</dc:creator>
	<dc:creator xml:lang="en">Triananinsi, Nurhidayat</dc:creator>
	<dc:creator xml:lang="en">Sampara, Nurqalbi</dc:creator>
	<dc:creator xml:lang="en">Sumarni, Sumarni</dc:creator>
	<dc:creator xml:lang="en">Minarti, Minarti</dc:creator>
	<dc:creator xml:lang="en">RA, A. Maya</dc:creator>
	<dc:subject xml:lang="en">DNA</dc:subject>
	<dc:subject xml:lang="en">mikrobiota usus</dc:subject>
	<dc:subject xml:lang="en">ASI</dc:subject>
	<dc:subject xml:lang="en">Sufor</dc:subject>
	<dc:description xml:lang="en">Breast milk is the best source of nutrition because it contains a variety of healthy compounds and immunity. Several studies of gut microbiota has proven its important role in the development of the immune system. This study aims to characterize the gut microbiota of babies born normally with breastfeeding and formula milk. This research using cross sectional study design with purposive sampling as sampling technique. Samples used were babies born normally as many as 30 infants that divided into two groups namely infants who given breast milk and combinated breastfeeding and formulamilk of 15 infants each. Stool samples were taken by swab technique, the DNA was extracted using a DNA kit continued DNA amplification by PCR with 16SrRNA universal primers then DNA sequencing. Data from DNA sequencer analized using a software of MEGA5 and BLAST attained on the NCBI link. The result showed that the group of infants who were breastfed plus formula milk had more varied intestinal microbiota than the group of infants who were only given breast milk. It can be concluded that intestinal microbiota that dominates the intestines of infants who are given breast milk can be a probiotic in the growth of the baby and the development of the immune system.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Public Health, Hasanuddin University, Makassar</dc:publisher>
	<dc:date>2020-03-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/mkmi/article/view/9050</dc:identifier>
	<dc:identifier>10.30597/mkmi.v16i1.9050</dc:identifier>
	<dc:source xml:lang="en">Media Kesehatan Masyarakat Indonesia; Vol. 16 No. 1: MARET 2020; 116-126</dc:source>
	<dc:source>2356-4067</dc:source>
	<dc:source>0216-2482</dc:source>
	<dc:source>10.30597/mkmi.v16i1</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/mkmi/article/view/9050/pdf</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2020 Mudyawati Kamaruddin</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>