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Optimization of Microplate Type Uv-vis Spectrophotometer Performance as an Antioxidant Activity Testing Instrument

Indonesian Journal of Chemical Science · Vol. 13 No. 1 (2024)

DOI
10.15294/ijcs.v13i1.5014
ISSN
2502-6844, 2252-6951
Terbit
2024-05-28
Penerbit
Prodi Kimia
Bahasa
eng

Abstrak

Eliminating a compound's capacity to impede and neutralize free radical-induced oxidation processes is the goal of antioxidant activity analysis. The DPPH (pikrilhidrazil-2,2-difenil-1) method was the approach taken in this investigation. Simple, sensitive, stable, quick, straightforward, and suitable for usage with tiny sample volumes are some of the benefits of DPPH procedures. In this investigation, some plant extracts were employed as a comparison, with ascorbic acid serving as the control. In order to evaluate a microplate-type uv-vis Spectrophotometer effectiveness as an antioxidant activity test tool, the study will determine its maximum wave, linearity, accuracy, and IC50 values. Wavelength selectivity is found in 517 nm. The results validation of the DPPH method for analyzing the activity of antioxidants. Measurements of the ascorbic acid solution as a control obtained R2 values of 0.9781 compared to the LoD and LoQ limits of 0.961 and 3.053 respectively. The IC50 of ascorbic acid, ginger and turmeric, respectively, was 14.7218 (strong), 29.9194 (very strong), and 52.4943 (strong).

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  • State University of Semarang
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Rekan penulisOrganisasiArtikel bersama
Ella KusumastutiAfiliasi tidak diketahui7
Mohammad AlauhdinAfiliasi tidak diketahui4
Nuni WidiartiState University of Semarang3
Cepi KurniawanState University of Semarang3
SRI WAHYUNIAfiliasi tidak diketahui3
Nanik WijayatiState University of Semarang2
Jumaeri JumaeriAfiliasi tidak diketahui2
dina herminaAfiliasi tidak diketahui2
Triastuti SulistyaningsihAfiliasi tidak diketahui2
Edy CahyonoState University of Semarang2
Indah PrasetyowatiAfiliasi tidak diketahui1
Eka IrawatiAfiliasi tidak diketahui1
Siti Herlina DewiState University of Semarang1
Mohammad Haris BaihaqiAfiliasi tidak diketahui1
Fika Maulydha HardiyantiAfiliasi tidak diketahui1
Eka Siti RinjaniAfiliasi tidak diketahui1
Sigit PriatmokoState University of Semarang1
Rr. Sri Endang PujiastutiAfiliasi tidak diketahui1
Sudarmin SudarminAfiliasi tidak diketahui1
Prasetyo ListiadjiAfiliasi tidak diketahui1
M. SasmitaAfiliasi tidak diketahui1
Dyah Rini IndriyantiState University of Semarang1
Samuel Budi Wardhana KusumaKanazawa University1
Saffanah Binti Mohd Ab AzidAfiliasi tidak diketahui1
Cindy MaharaniAfiliasi tidak diketahui1
Prayogo Probo AsmoroAfiliasi tidak diketahui1
Rizka Ayu MelykhatunAfiliasi tidak diketahui1
HarjonoState University of Semarang1
Riska Putri MawarniAfiliasi tidak diketahui1
RatnawatiAfiliasi tidak diketahui1
Syakilla Adha NandiantaAfiliasi tidak diketahui1
Latifah LatifahAfiliasi tidak diketahui1
Atika RapiernaAfiliasi tidak diketahui1
Hayyu FitriahAfiliasi tidak diketahui1
Eko Budi SusatyoAfiliasi tidak diketahui1
Eka HartantiAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/ijcs/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 5 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Optimization of Microplate Type Uv-vis  Spectrophotometer Performance as an Antioxidant Activity Testing Instrument</dc:title>
	<dc:creator xml:lang="en">Martin Sulistyani</dc:creator>
	<dc:creator xml:lang="en">F. Widhi Mahatmanti</dc:creator>
	<dc:creator xml:lang="en">Nuril Huda</dc:creator>
	<dc:creator xml:lang="en">Ridho Prasetyo</dc:creator>
	<dc:subject xml:lang="en">Method Validation, Antioxidants, DPPH (2,2-difenil-1- pikrilhidrazil)</dc:subject>
	<dc:description xml:lang="en">Eliminating a compound's capacity to impede and neutralize free radical-induced oxidation processes is the goal of antioxidant activity analysis. The DPPH (pikrilhidrazil-2,2-difenil-1) method was the approach taken in this investigation. Simple, sensitive, stable, quick, straightforward, and suitable for usage with tiny sample volumes are some of the benefits of DPPH procedures. In this investigation, some plant extracts were employed as a comparison, with ascorbic acid serving as the control. In order to evaluate a microplate-type uv-vis  Spectrophotometer effectiveness as an antioxidant activity test tool, the study will determine its maximum wave, linearity, accuracy, and IC50 values. Wavelength selectivity is found in 517 nm. The results validation of the DPPH method for analyzing the activity of antioxidants. Measurements of the ascorbic acid solution as a control obtained R2 values of 0.9781 compared to the LoD and LoQ limits of 0.961 and 3.053 respectively. The IC50 of ascorbic acid, ginger and turmeric, respectively, was 14.7218 (strong), 29.9194 (very strong), and 52.4943 (strong). </dc:description>
	<dc:publisher xml:lang="en">Prodi Kimia</dc:publisher>
	<dc:date>2024-05-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/ijcs/article/view/5014</dc:identifier>
	<dc:identifier>10.15294/ijcs.v13i1.5014</dc:identifier>
	<dc:source xml:lang="en">Indonesian Journal of Chemical Science; Vol. 13 No. 1 (2024)</dc:source>
	<dc:source>2502-6844</dc:source>
	<dc:source>2252-6951</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/ijcs/article/view/5014/452</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Indonesian Journal of Chemical Science</dc:rights>
</oai_dc:dc>