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Aktivitas Antioksidan Hidrolisat Kolagen Kulit Ikan Nila (Oreochromis niloticus): Antioxidant activity of Tilapia (Oreochromis niloticus) Skin Collagen Hydrolizate

Jurnal Pengolahan Hasil Perikanan Indonesia · Vol. 23 No. 3 (2020) · pp. 423–433

DOI
10.17844/jphpi.v23i3.31732
ISSN
2354-886X, 2303-2111
Published
2020-11-24
Publisher
Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)
Language
ind

Abstract

Indonesian

Tilapia skin is a by-product of fish processing which can be used as an alternative raw material for collagen and its hydrolyzate. Collagen hydrolyzate is known to have potential biological activities including antioxidants. This study aims to examine the antioxidant activity of collagen hydrolyzate from the skin of tilapia (Oreochromis niloticus) in vitro. This research was divided into three stages including collagen pretratment, collagen extraction and collagen hydrolysis. In the hydrolysis process papain enzymes are used with different concentrations (4,000 U/g, 6,000 U/g, 8,000 U/g samples) with hydrolysis time for 60, 120 and 180 minutes. The average yield value of tilapia skin collagen hydrolyzate was 15.17%. The hydrolysis time, the concentration of enzymes given and the interaction between the two factors significantly affected the value of the degree of hydrolysis and antioxidant activity of the hydrolyzed collagen of tilapia skin. Collagen hydrolyzed for 120 minutes by giving an enzyme concentration of 8,000 U had an average percentage of degree of hydrolysis of 33.94%, and the best IC50 value was 93.32 µg/mL, categorized as strong antioxidant compounds. The zeta potential value of the tilapia skin collagen hydrolyzate was -10.9 mV.

English

Kulit ikan nila merupakan hasil samping proses pengolahan ikan yang dapat dimanfaatkan sebagai alternatif bahan baku kolagen dan hidrolisatnya. Hidrolisat kolagen diketahui memiliki aktivitas biologis yang potensial diantaranya sebagai antioksidan. Penelitian ini bertujuan untuk menentukan aktivitas antioksidan hidrolisat kolagen dari kulit ikan nila (Oreochromis niloticus) secara in vitro. Penelitian ini terbagi menjadi tiga tahap meliputi fase praperlakuan kolagen, ekstraksi kolagen dan hidrolisis kolagen. Pada proses hidrolisis digunakan enzim papain dengan konsentrasi yang berbeda (4.000 U/g, 6.000 U/g, 8.000 U/g sampel) dengan waktu hidrolisis selama 60, 120 dan 180 menit. Rerata nilai rendemen hidrolisat kolagen kulit ikan nila sebesar 15,17%. Waktu hidrolisis, konsentrasi enzim yang diberikan serta interaksi antar kedua faktor tersebut berpengaruh secara signifikan terhadap nilai derajat hidrolisis dan aktivitas antioksidan hidrolisat kolagen kulit ikan nila. Hidrolisat kolagen kulit ikan nila yang dihidrolisis selama 120 menit dengan pemberian konsentrasi enzim sebesar 8.000 U memiliki rerata persentase derajat hidrolisis sebesar 33,94%, serta rerata nilai IC50 terbaik yakni 93,32 µg/mL, termasuk pada kategori senyawa antioksidan kuat. Nilai zeta potensial hidrolisat kolagen kulit ikan nila adalah -10,9 mV.

Keywords

Collaboration network of the authors

40 authors · 209 co-authorship links · 13 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • IPB University
  • Bandung Institute of Technology
  • Badan Pengkajian dan Penerapan Teknologi
  • Universitas Halu Oleo
  • Universitas Pasir Pangaraian
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Agoes Mardiono JacoebIPB University11
Bambang RiyantoIPB University10
‪Tati NurhayatiIPB University6
Wahyu RamadhanIPB University5
Joko SantosoIPB University5
Iriani SetyaningsihIPB University5
Ruddy SuwandiIPB University4
Asadatun AbdullahIPB University4
Mala NurilmalaIPB University4
Pipih SuptijahIPB University4
Tati NurhayatiIPB University4
Sugeng Heri SusenoIPB University4
Joko SantosoIPB University3
Bambang RiyantoBandung Institute of Technology3
Bustami IbrahimIPB University3
Taufik HidayatBadan Pengkajian dan Penerapan Teknologi3
Kustiariyah TarmanIPB University3
Farel WidiarsyahIPB University2
Silva FauziahIPB University2
Fahri SinulinggaIPB University2
. UjuAffiliation unknown2
NurokhmatunnisaIPB University2
Detti - PujiantiIPB University2
Rizsa Mustika PertiwiIPB University2
Wahyu RamadhanAffiliation unknown2
Mala NurilmalaAffiliation unknown2
Ratri IsharyadiUniversitas Pasir Pangaraian1
Zacky Arivai SantosaeAffiliation unknown1
Wahyu RamadhanIPB University1
Purnama ArafahIPB University1
Tri WahyuningsihIPB University1
Muhammad NurdinUniversitas Halu Oleo1
ZuriatAffiliation unknown1
Safrina Dyah HardiningtyasIPB University1
Cahyuning IsnainiIPB University1
Zacky Arivaie SantosaIPB University1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/jphpi/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="id">Aktivitas Antioksidan Hidrolisat Kolagen Kulit  Ikan Nila (Oreochromis niloticus): Antioxidant activity of Tilapia (Oreochromis niloticus) Skin Collagen Hydrolizate</dc:title>
	<dc:creator xml:lang="id">Prastyo, Deny Tri</dc:creator>
	<dc:creator xml:lang="id">Trilaksani, Wini</dc:creator>
	<dc:creator xml:lang="id">Nurjanah</dc:creator>
	<dc:creator xml:lang="en">Prastyo, Deny Tri</dc:creator>
	<dc:creator xml:lang="en">Trilaksani, Wini</dc:creator>
	<dc:creator xml:lang="en">Nurjanah</dc:creator>
	<dc:subject xml:lang="en">ABTS</dc:subject>
	<dc:subject xml:lang="en">enzim papain</dc:subject>
	<dc:subject xml:lang="en">derajat hidrolisis</dc:subject>
	<dc:subject xml:lang="en">zeta potensial</dc:subject>
	<dc:subject xml:lang="id">ABTS</dc:subject>
	<dc:subject xml:lang="id">degree of hydrolysis</dc:subject>
	<dc:subject xml:lang="id">papain enzyme</dc:subject>
	<dc:subject xml:lang="id">zeta potential</dc:subject>
	<dc:description xml:lang="id">Tilapia skin is a by-product of fish processing which can be used as an alternative raw material for collagen and its hydrolyzate. Collagen hydrolyzate is known to have potential biological activities including antioxidants. This study aims to examine the antioxidant activity of collagen hydrolyzate from the skin of tilapia (Oreochromis niloticus) in vitro. This research was divided into three stages including collagen pretratment, collagen extraction and collagen hydrolysis. In the hydrolysis process papain enzymes are used with different concentrations (4,000 U/g, 6,000 U/g, 8,000 U/g samples) with hydrolysis time for 60, 120 and 180 minutes. The average yield value of tilapia skin collagen hydrolyzate was 15.17%. The hydrolysis time, the concentration of enzymes given and the interaction between the two factors significantly affected the value of the degree of hydrolysis and antioxidant activity of the hydrolyzed collagen of tilapia skin. Collagen hydrolyzed for 120 minutes by giving an enzyme concentration of 8,000 U had an average percentage of degree of hydrolysis of 33.94%, and the best IC50 value was 93.32 µg/mL, categorized as strong antioxidant compounds. The zeta potential value of the tilapia skin collagen hydrolyzate was -10.9 mV.</dc:description>
	<dc:description xml:lang="en">Kulit ikan nila merupakan hasil samping proses pengolahan ikan yang dapat dimanfaatkan sebagai alternatif bahan baku kolagen dan hidrolisatnya. Hidrolisat kolagen diketahui memiliki aktivitas biologis yang potensial diantaranya sebagai antioksidan. Penelitian ini bertujuan untuk menentukan aktivitas antioksidan hidrolisat kolagen dari kulit ikan nila (Oreochromis niloticus) secara in vitro. Penelitian ini terbagi menjadi tiga tahap meliputi fase praperlakuan kolagen, ekstraksi kolagen dan hidrolisis kolagen. Pada proses hidrolisis digunakan enzim papain dengan konsentrasi yang berbeda (4.000 U/g, 6.000 U/g, 8.000 U/g sampel) dengan waktu hidrolisis selama 60, 120 dan 180 menit. Rerata nilai rendemen hidrolisat kolagen kulit ikan nila sebesar 15,17%. Waktu hidrolisis, konsentrasi enzim yang diberikan serta interaksi antar kedua faktor tersebut berpengaruh secara signifikan terhadap nilai derajat hidrolisis dan aktivitas antioksidan hidrolisat kolagen kulit ikan nila. Hidrolisat kolagen kulit ikan nila yang dihidrolisis selama 120 menit dengan pemberian konsentrasi enzim sebesar 8.000 U memiliki rerata persentase derajat hidrolisis sebesar 33,94%, serta rerata nilai IC50 terbaik yakni 93,32 µg/mL, termasuk pada kategori senyawa antioksidan kuat. Nilai zeta potensial hidrolisat kolagen kulit ikan nila adalah -10,9 mV.</dc:description>
	<dc:publisher xml:lang="en">Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)</dc:publisher>
	<dc:date>2020-11-24</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
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	<dc:identifier>https://journal.ipb.ac.id/jphpi/article/view/31732</dc:identifier>
	<dc:identifier>10.17844/jphpi.v23i3.31732</dc:identifier>
	<dc:source xml:lang="id">Jurnal Pengolahan Hasil Perikanan Indonesia; Vol 23 No 3 (2020): Jurnal Pengolahan Hasil Perikanan Indonesia 23(3); 423-433</dc:source>
	<dc:source xml:lang="en">Jurnal Pengolahan Hasil Perikanan Indonesia; Vol. 23 No. 3 (2020): Jurnal Pengolahan Hasil Perikanan Indonesia 23(3); 423-433</dc:source>
	<dc:source>2354-886X</dc:source>
	<dc:source>2303-2111</dc:source>
	<dc:language>ind</dc:language>
	<dc:relation>https://journal.ipb.ac.id/jphpi/article/view/31732/20711</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2020 Deny Tri Prastyo, Wini Trilaksani, Nurjanah</dc:rights>
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