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Karakteristik Kolagen dari Kulit dan Sisik Ikan Coklatan, Swanggi, dan Kurisi sebagai Bahan Gelatin: Characteristics of Collagen Skin and Scales of Lattice Monocle Bream, Purple-Spotted Bigeye and Threadfin Bream as Gelatin Ingredients

Jurnal Pengolahan Hasil Perikanan Indonesia · Vol. 25 No. 3 (2022) · pp. 512–527

DOI
10.17844/jphpi.v25i3.43598
ISSN
2354-886X, 2303-2111
Published
2022-12-30
Publisher
Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)
Language
ind

Abstract

Indonesian

Hasil samping industri filet ikan coklatan, swanggi dan kurisi berupa kulit, yang hanya dimanfaatkan sebagai bahan pakan ternak meskipun dapat dimanfaatkan sebagai sumber kolagen dan gelatin. Kandungan hidroksiprolina pembentuk kolagen beserta sifat protein kolagen belum tersedia informasinya, sehingga dilakukan penelitian. Penelitian bertujuan menentukan jenis kulit terpilih sebagai bahan gelatin dengan memperhatikan karakteristik kulit, sisik, dan kulit bersisik ikan coklatan, swanggi, kurisi, dan kandungan hidoksiprolina sebagai penciri kolagen. Rancangan percobaan yang digunakan pada tahap deproteinasi adalah menggunakan Rancangan Acak Kelompok (RAK) tiga faktor di mana faktor pertama yaitu jenis ikan terdiri atas tiga taraf (ikan coklatan, swanggi, dan kurisi), faktor kedua yaitu waktu perendaman NaOH 12 taraf (2; 4; 6; 8; 10; 12; 14; 16; 18; 20; 22; 24) jam, faktor ketiga jenis kulit tiga taraf (kulit, sisik, kulit bersisik). Data dianalisis secara statistik dengan ANOVA dan uji lanjut Duncan. Hasil penelitian menunjukkan bahwa waktu optimasi deproteinasi perendaman NaOH dimulai dari 14-20 jam tergantung jenis ikan dan bahan baku, efektif mengeliminasi protein non kolagen. Luas jaringan kolagen pengamatan histologi adalah ±95,136,16 µm² tertinggi pada jenis kulit bersisik ikan swanggi berasosiasi dengan kandungan protein tertinggi pada kulit bersisik swanggi sebesar 21,20 % (basis basah) 56,17 % (basis kering). Jenis kulit memiliki berat molekul dalam kisaran 10 sampai 105 kDa mempunyai kandungan protein yang merupakan protein sarkoplasma, miofibril, dan jaringan ikat yang mengandung kolagen, sehingga dapat digunakan sebagai penghasil gelatin. Kulit bersisik ikan swanggi memiliki kandungan hidroksiprolina tertinggi daripada jenis kulit lainnya yaitu 0,0888 g/µL sehingga dapat ditentukan sebagai jenis kulit terpilih.

English

The by-product of the fish fillet industry for lattice monocle bream, purple-spotted bigeye and threadfin bream, are only used as an ingredient for animal feed although it can be used as a source of collagen and gelatin. The hydroxyproline content forming collagen along with the properties of collagen protein is not yet available, so research is done. This study was aimed to determine the selected skin type as a gelatin material by taking into account the characteristics of the skin and scales of lattice monocle bream, purple-spotted bigeye, threadfin bream, and hydroxyproline content as a collagen marker. The experimental design used at the deproteination stage used a Randomized Block Design (RBD) with three factors where the first factor was the type of fish consisting of three levels (lattice monocle bream, purple-spotted bigeye and threadfin bream), the second factor was the soaking time of NaOH 12 levels (2; 4; 6; 8; 10; 12; 14; 16; 18; 20; 22; 24) hours, the third factor of the skin type is three levels (skin, scales, scaly skin), The experimental design in this study was analyzed by statistic with ANOVA and Duncan’s advanced test. The results showed that the deproteinization optimization time of NaOH immersion started from 14-20 hours depending on the type of fish and the raw materials, effectively eliminated non-collagen proteins. The area of collagen tissue in histology test preparations was ±95.136,16 µm² highest in the scaly skin of the purple-spotted bigeye associated with the highest protein content in purple-spotted bigeye scaly skin of 21.20±0.18% (wet basis) 56.17±0.12% (dry basis). The skin type had a molecular weight in the range of 10 to 105 kDa. The raw material contains protein which is sarcoplasmic protein, myofibrils and collagen containing connective tissue, so the raw material can be used as a gelatin producer. The scaly skin of purple spotted bream has the highest hydroxyproline than other skin types of 0.0888 µg/µL, so it can be determined as the selected skin types.

Keywords

Collaboration network of the authors

40 authors · 140 co-authorship links · showing the 40 most connected

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  • IPB University
  • State University of Semarang
  • Affiliation unknown
  • Different organisations
  • Same organisation
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Co-authorOrganisationJoint articles
Tati NurhayatiAffiliation unknown11
Asadatun AbdullahAffiliation unknown7
Pipih SuptijahIPB University7
Rizsa Mustika PertiwiAffiliation unknown5
Arief YuliantoState University of Semarang4
Iriani SetyaningsihIPB University4
Roni NugrahaAffiliation unknown4
nurjanah nurjanahAffiliation unknown4
Joko SantosoAffiliation unknown4
Wijang SakitriAffiliation unknown3
Sucihatiningsih Dian Wisika PrajantiState University of Semarang3
Partono ThomasAffiliation unknown3
NurjanahAffiliation unknown3
Taufik HidayatAffiliation unknown3
Sugeng Heri SusenoIPB University3
Agoes Mardiono JacoebAffiliation unknown3
Wahyu RamadhanAffiliation unknown3
Wini TrilaksaniIPB University3
Kustiariyah TarmanIPB University3
Slamet SumartoAffiliation unknown2
Martitah MartitahAffiliation unknown2
Ahmad SehabuddinAffiliation unknown2
Jarot Tri Bowo SantosoAffiliation unknown2
Joko WidodoAffiliation unknown2
Rusdarti RusdartiAffiliation unknown2
Heri YantoAffiliation unknown2
Ketut SudarmaAffiliation unknown2
Arip WijiantoAffiliation unknown2
Nuri AndarwulanAffiliation unknown2
MAGGY T. SUHARTONOAffiliation unknown2
Lily VirulyAffiliation unknown2
medal lintas percekaAffiliation unknown2
Desniar DesniarAffiliation unknown2
Safrina Dyah HardiningtyasIPB University2
Cecep KusmanaAffiliation unknown1
Majariana KrisantiAffiliation unknown1
Sigid HariyadiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/jphpi/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="id">Karakteristik Kolagen dari Kulit dan Sisik Ikan Coklatan, Swanggi, dan Kurisi sebagai Bahan Gelatin: Characteristics of Collagen Skin and Scales of Lattice Monocle Bream, Purple-Spotted Bigeye and Threadfin Bream as Gelatin Ingredients</dc:title>
	<dc:creator xml:lang="id">Widiyanto, Widiyanto</dc:creator>
	<dc:creator xml:lang="id">Uju, Uju</dc:creator>
	<dc:creator xml:lang="id">Nurilmala, Mala</dc:creator>
	<dc:subject xml:lang="en">deproteinization</dc:subject>
	<dc:subject xml:lang="en">histology</dc:subject>
	<dc:subject xml:lang="en">hydroxyproline</dc:subject>
	<dc:subject xml:lang="en">SDS-PAGE</dc:subject>
	<dc:subject xml:lang="id">deproteinasi</dc:subject>
	<dc:subject xml:lang="id">hidroksiprolina</dc:subject>
	<dc:subject xml:lang="id">histologi</dc:subject>
	<dc:subject xml:lang="id">SDS-PAGE</dc:subject>
	<dc:description xml:lang="id">Hasil samping industri filet ikan coklatan, swanggi dan kurisi berupa kulit, yang hanya dimanfaatkan sebagai bahan pakan ternak meskipun dapat dimanfaatkan sebagai sumber kolagen dan gelatin. Kandungan hidroksiprolina pembentuk kolagen beserta sifat protein kolagen belum tersedia informasinya, sehingga dilakukan penelitian. Penelitian bertujuan menentukan jenis kulit terpilih sebagai bahan gelatin dengan memperhatikan karakteristik kulit, sisik, dan kulit bersisik ikan coklatan, swanggi, kurisi, dan kandungan hidoksiprolina sebagai penciri kolagen. Rancangan percobaan yang digunakan pada tahap deproteinasi adalah menggunakan Rancangan Acak Kelompok (RAK) tiga faktor di mana faktor pertama yaitu jenis ikan terdiri atas tiga taraf (ikan coklatan, swanggi, dan kurisi), faktor kedua yaitu waktu perendaman NaOH 12 taraf (2; 4; 6; 8; 10; 12; 14; 16; 18; 20; 22; 24) jam, faktor ketiga jenis kulit tiga taraf (kulit, sisik, kulit bersisik). Data dianalisis secara statistik dengan ANOVA dan uji lanjut Duncan. Hasil penelitian menunjukkan bahwa waktu optimasi deproteinasi perendaman NaOH dimulai dari 14-20 jam tergantung jenis ikan dan bahan baku, efektif mengeliminasi protein non kolagen. Luas jaringan kolagen pengamatan histologi adalah ±95,136,16 µm² tertinggi pada jenis kulit bersisik ikan swanggi berasosiasi dengan kandungan protein tertinggi pada kulit bersisik swanggi sebesar 21,20 % (basis basah) 56,17 % (basis kering). Jenis kulit memiliki berat molekul dalam kisaran 10 sampai 105 kDa mempunyai kandungan protein yang merupakan protein sarkoplasma, miofibril, dan jaringan ikat yang mengandung kolagen, sehingga dapat digunakan sebagai penghasil gelatin. Kulit bersisik ikan swanggi memiliki kandungan hidroksiprolina tertinggi daripada jenis kulit lainnya yaitu 0,0888 g/µL sehingga dapat ditentukan sebagai jenis kulit terpilih.</dc:description>
	<dc:description xml:lang="en">The by-product of the fish fillet industry for lattice monocle bream, purple-spotted bigeye and threadfin bream, are only used as an ingredient for animal feed although it can be used as a source of collagen and gelatin. The hydroxyproline content forming collagen along with the properties of collagen protein is not yet available, so research is done. This study was aimed to determine the selected skin type as a gelatin material by taking into account the characteristics of the skin and scales of lattice monocle bream, purple-spotted bigeye, threadfin bream, and hydroxyproline content as a collagen marker. The experimental design used at the deproteination stage used a Randomized Block Design (RBD) with three factors where the first factor was the type of fish consisting of three levels (lattice monocle bream, purple-spotted bigeye and threadfin bream), the second factor was the soaking time of NaOH 12 levels (2; 4; 6; 8; 10; 12; 14; 16; 18; 20; 22; 24) hours, the third factor of the skin type is three levels (skin, scales, scaly skin), The experimental design in this study was analyzed by statistic with ANOVA and Duncan’s advanced test. The results showed that the deproteinization optimization time of NaOH immersion started from 14-20 hours depending on the type of fish and the raw materials, effectively eliminated non-collagen proteins. The area of collagen tissue in histology test preparations was ±95.136,16 µm² highest in the scaly skin of the purple-spotted bigeye associated with the highest protein content in purple-spotted bigeye scaly skin of 21.20±0.18% (wet basis) 56.17±0.12% (dry basis). The skin type had a molecular weight in the range of 10 to 105 kDa. The raw material contains protein which is sarcoplasmic protein, myofibrils and collagen containing connective tissue, so the raw material can be used as a gelatin producer. The scaly skin of purple spotted bream has the highest hydroxyproline than other skin types of 0.0888 µg/µL, so it can be determined as the selected skin types.</dc:description>
	<dc:publisher xml:lang="en">Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)</dc:publisher>
	<dc:date>2022-12-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/jphpi/article/view/43598</dc:identifier>
	<dc:identifier>10.17844/jphpi.v25i3.43598</dc:identifier>
	<dc:source xml:lang="id">Jurnal Pengolahan Hasil Perikanan Indonesia; Vol 25 No 3 (2022): Jurnal Pengolahan Hasil Perikanan Indonesia 25(3); 512-527</dc:source>
	<dc:source xml:lang="en">Jurnal Pengolahan Hasil Perikanan Indonesia; Vol. 25 No. 3 (2022): Jurnal Pengolahan Hasil Perikanan Indonesia 25(3); 512-527</dc:source>
	<dc:source>2354-886X</dc:source>
	<dc:source>2303-2111</dc:source>
	<dc:language>ind</dc:language>
	<dc:relation>https://journal.ipb.ac.id/jphpi/article/view/43598/24906</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2022 Widiyanto Widiyanto, Uju Uju, Mala Nurilmala</dc:rights>
</oai_dc:dc>