PENGARUH CARA PENGUKUSAN DAN PENGERINGAN TERHADAP KANDUNGAN SENYAWA FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN TERIPANG KELING (Holothuria atra) DARI PERAIRAN DESA ULUSAWA, KECAMATAN LAONTI, KABUPATEN KONAWE SELATAN

Abstract

ABSTRACT The aim of this study was to determine the proximate analysis, phytochemical, and antioxidant value of keling sea cucumbers with different processing methods (steaming at 100oC and drying at 60oC). The parameters tested were proximate analysis (water, ash, protein, fat and carbohydrate content), phytochemicals test (alkaloids, flavonoids, tannins, steroids, triterpenoids and saponins), and antioxidant activity. The results of this study obtained that the highest proximate values of water and carbohydrate content were found in the treatment of fresh sea cucumbers with values of 69.52% and 16.39%, respectivelly. For ash, protein and fat content, were found in the treatment of dried sea cucumbers with values of 10.94%, 53.54%, and 35.07% respectivelly. Phytochemical test of ethanol extract of sea cucumber (Holothuria atra) containing saponins, steroids and triterpenoids. The antioxidant activity test of sea cucumbers contained IC50 values ranging from 4616.35 ppm-3785.92 ppm, the best IC50 value was found in the fresh treatment with a value of 3785.92 ppm.Keywords: Antioxidant, keling, phytochemical, processing, proximate.ABSTRAKTujuan dari penelitian ini adalah untuk mengetahui nilai proksimat, nilai fitokimia dan nilai antioksidan teripang keling dengan cara pengolahan yang berbeda (pengukusan suhu 100oC dan pengeringan 60oC). Parameter yang diuji yaitu proksimat (Kadar air, kadar abu, kadar protein, kadar lemak dan kadar karbohidrat), fitokimia (Alkaloid, flavonoid, tanin, steroid, triterpenoid dan saponin), dan aktivitas antioksidan. Hasil penelitian ini diperoleh nilai proksimat tertinggi kadar air dan kadar karbohidrat terdapat pada perlakuan teripang segar dengan nilai 69,52% dan 16,39%. Kadar abu, kadar protein dan kadar lemak tertinggi terdapat pada perlakuan teripang kering dengan nilai 10,94%, 53,54%, dan 35,07%. Uji fitokimia ekstrak etanol teripang keling (Holothuria atra) mengandung senyawa saponin, steroid dan triterpenoid. Uji aktivitas antioksidan teripang terdapat nilai IC50 berkisar antara 4616,35 ppm-3785,92 ppm, nilai IC50 terendah terdapat pada perlakuan segar dengan memiliki nilai 3785,92 ppm.Kata kunci: Antioksidan, fitokimia, pengolahan, proksimat, teripang.