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Isolation and caracterization of ficin enzyme from Ficus septica Burm F stem latex

Indonesian Journal of Biotechnology · Vol. 20 No. 2 (2015) · hlm. 161–166

DOI
10.22146/ijbiotech.24200
ISSN
2089-2241, 0853-8654
Terbit
2015-12-01
Penerbit
Universitas Gadjah Mada
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

This research aims to isolate and characterize the fcin enzyme from Ficus septica stem latex. Ficin from Ficus septica stem latex was isolated using column chromatography. Then enzyme activity was tested at different temperature (40oC, 50oC, 60oC, 70oC) and pH (6.0, 7.0, 8.0) levels. Ficin enzyme activity of joint treatment with variations in temperature and pH was analyzed using two-way ANOVA with a factorial pattern followed by Least Signifcant Difference (LSD) test. The results showed that temperature treatment signifcantly affects enzyme activity. However, the treatment of pH and the interaction between temperature and pH did not signifcantly affect the fcin enzyme activity. There was no signifcant difference in fcin activity at the incubation temperatures of 40oC and 50oC, as well as 60oC and 70oC. However, comparing the incubation temperatures of 40oC and 50oC with treatment 60°C and 70°C showed a signifcant difference in fcin enzyme activity. In the treatment of incubation at pH 6, 7 and 8 for fcin enzyme activity showed no signifcant difference. We concluded that the Ficus septica plant latex contained fcin enzyme with an optimum temperature of 60°C and optimum pH of 6, 7, and 8.

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  • State University of Semarang
  • Universitas Halu Oleo
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Rekan penulisOrganisasiArtikel bersama
Muhammad Syukri SadimantaraUniversitas Halu Oleo15
SRI REJEKIUniversitas Halu Oleo15
Ari YuniastutiState University of Semarang12
Andi KhaeruniUniversitas Halu Oleo10
RH Fitri FaradillaAfiliasi tidak diketahui10
Ansharullah AnsharullahUniversitas Halu Oleo10
Hermanto HermantoAfiliasi tidak diketahui8
Nur AsyikUniversitas Halu Oleo6
Asnani AsnaniAfiliasi tidak diketahui6
Siti Harnina BintariAfiliasi tidak diketahui6
Tamrin TamrinAfiliasi tidak diketahui5
Sri KadarwatiState University of Semarang5
La KarimunaAfiliasi tidak diketahui4
Rudi HartonoAfiliasi tidak diketahui4
Sigit PriatmokoState University of Semarang4
Muchamad DafipAfiliasi tidak diketahui4
Aditya MariantiAfiliasi tidak diketahui4
Fitri Arum SasiAfiliasi tidak diketahui4
Sarinah SarinahAfiliasi tidak diketahui3
Prima Endang SusilowatiAfiliasi tidak diketahui3
R. Susanti R. SusantiAfiliasi tidak diketahui3
Didik Huswo UtomoAfiliasi tidak diketahui3
Subiyanto Hadi SaputroAfiliasi tidak diketahui3
Jumaeri JumaeriAfiliasi tidak diketahui3
Andreas Priyono Budi PrasetyoAfiliasi tidak diketahui3
Sri SukaesihState University of Semarang3
Zuraidah ZuraidahAfiliasi tidak diketahui3
Sigit SaptonoState University of Semarang3
Enni Suwarsi RahayuState University of Semarang3
Yustinus Ulung AnggraitoAfiliasi tidak diketahui3
Thamrin AzisAfiliasi tidak diketahui2
Mashuni MashuniAfiliasi tidak diketahui2
Muhammad Zakir MuzakkarAfiliasi tidak diketahui2
Abdu Rahman BacoAfiliasi tidak diketahui2
Kobajashi Togo IsamuAfiliasi tidak diketahui2
Safrika SafrikaAfiliasi tidak diketahui1
Bagio BagioAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Isolation and caracterization of ficin enzyme from Ficus septica Burm F stem latex</dc:title>
	<dc:creator>Wahyuni, Sri</dc:creator>
	<dc:creator>Susanti, R.</dc:creator>
	<dc:creator>Iswari, Retno Sri</dc:creator>
	<dc:subject xml:lang="en-US">Ficus septica Burm F; ficin enzyme; temperature; pH</dc:subject>
	<dc:description xml:lang="en-US">This research aims to isolate and characterize the fcin enzyme from Ficus septica stem latex. Ficin from Ficus septica stem latex was isolated using column chromatography. Then enzyme activity was tested at different temperature (40oC, 50oC, 60oC, 70oC) and pH (6.0, 7.0, 8.0) levels. Ficin enzyme activity of joint treatment with variations in temperature and pH was analyzed using two-way ANOVA with a factorial pattern followed by Least Signifcant Difference (LSD) test. The results showed that temperature treatment signifcantly affects enzyme activity. However, the treatment of pH and the interaction between temperature and pH did not signifcantly affect the fcin enzyme activity. There was no signifcant difference in fcin activity at the incubation temperatures of 40oC and 50oC, as well as 60oC and 70oC. However, comparing the incubation temperatures of 40oC and 50oC with treatment 60°C and 70°C showed a signifcant difference in fcin enzyme activity. In the treatment of incubation at pH 6, 7 and 8 for fcin enzyme activity showed no signifcant difference. We concluded that the Ficus septica plant latex contained fcin enzyme with an optimum temperature of 60°C and optimum pH of 6, 7, and 8.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2015-12-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/24200</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.24200</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 20, No 2 (2015); 161-166</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/24200/15815</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 Indonesian Journal of Biotechnology</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>